Artificial three-dimensional niches deconstruct pancreas development in vitro.
نویسندگان
چکیده
In the context of a cellular therapy for diabetes, methods for pancreatic progenitor expansion and subsequent differentiation into insulin-producing beta cells would be extremely valuable. Here we establish three-dimensional culture conditions in Matrigel that enable the efficient expansion of dissociated mouse embryonic pancreatic progenitors. By manipulating the medium composition we generate either hollow spheres, which are mainly composed of pancreatic progenitors, or complex organoids that spontaneously undergo pancreatic morphogenesis and differentiation. The in vitro maintenance and expansion of pancreatic progenitors require active Notch and FGF signaling, thus recapitulating in vivo niche signaling interactions. Our experiments reveal new aspects of pancreas development, such as a community effect by which small groups of cells better maintain progenitor properties and expand more efficiently than isolated cells, as well as the requirement for three-dimensionality. Finally, growth conditions in chemically defined biomaterials pave the way for testing the biophysical and biochemical properties of the niche that sustains pancreatic progenitors.
منابع مشابه
P-90: The Effect of Nitric Oxide on Mouse Oocyte in Vitro Maturation in Two and Three Dimensional Conditions
Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...
متن کاملHuman Coagulated Plasma as a Natural and Low Cost Matrix for in vitro Angiogenesis
Background: Angiogenesis, the development of new blood vessels, is an important process in tissue development and wound healing, but becomes pathologic when associated with solid tumor growth, proliferative retinopathies, and rheumatoid arthritis. Accurate and reliable qualification of neovascular (angiogenic) response, both in vitro and in vivo, is an essential requirement for the study of new...
متن کاملThree-dimensional characteristic approach for incompressible thermo-flows and influence of artificial compressibility parameter
In this paper the characteristics of unsteady three-dimensional incompressible flows with heat transfer are obtained along with artificial compressibility of Chorin. At first, compatibility equations and pseudo characteristics for three-dimensional flows are derived from five governing equations (continuity equation, Momentum equations in three directions, and energy equation) and then results ...
متن کاملO-16: Comparison of Pre-Antral Follicle Culture Development during 2 Dimensional and 3 Dimensional Culture Systems
Background: Setting up an in vitro follicle culture system that resembles in vivo ovary condition has high value in research. Additionally, expression evaluation of folliculogenesis involved genes could lead us to the designing of better culture system. Materials and Methods: ovaries of 12-day-old female NMRI mice were removed, 100-130 μm pre-antral follicles were mechanically isolated from fre...
متن کاملA Review on Application of Three Dimensional Culture and Testicular Scaffolds to Induction of in-Vitro Spermatogenesis
Introduction: Induction of in vitro spermatogenesis can be useful for infertility treatment in azoospermic patients and those undergoing chemotherapy. Different culture systems have been used to achieve this goal. This review study was performed with the aim to evaluate the application of 3D culture and testicular scaffolds in the establishment of in vitro spermatogenesis. In this review study...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Development
دوره 140 21 شماره
صفحات -
تاریخ انتشار 2013